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Further, the observed lethality at all temperatures and in the absence of DNA damage in mre11-nuclease minus dna2Δ pif1Δ expressing nuclease defective Dna2 leads to the hypothesis that Mre11 nuclease may play a role in Okazaki fragment processing or in repair of lethal endogenous DNA damage.
The median lethal dose (LD50) was calculated as geometric mean of the dose that resulted in 100%% lethality and the maximum dose with no lethality at all.
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Polδ absence caused lethality at an earlier embryonic stage than these aforementioned DNA polymerases.
Thus, this analysis uncovered unanticipated limitations regarding rad61 bypass of Ctf7 function but confirm rad61-bypass of ctf7 null cell lethality at a wide range of temperatures.
PU.1 mutants lead to embryonic lethality at a late gestational stage (Scott et al, 1994).
Interestingly, RAL-237, which carries the long opa35 rare variant allele, had the lowest embryonic lethality at 3.7% of all six lines tested.
As summarized in Table 5, mibefradil (T-type) showed the highest lethal activity (36.8±5.7% lethality at 1 mM) followed by verapamil (L-type, 24.3±5.1% lethality).
To induce strong dCREG depletion, we shifted UAS-CREG-RNAi; da-GAL4 flies cultivated at 18 °C to 25 °C, resulting in lethality at late pupal stage (only 3% of all pupae eclosed).
It is feared by all because of its 100% lethality at close range.
Conventional gene-targeting of pten in all mouse tissues (pten−/− mice) results in developmental delay and lethality at embryonic days 6.5 8.5 due to a failure in chorio-allantoic fusion (Kishimoto et al, 2003).
Systemic deletion of Yap results in embryonic lethality at day E8.5 [145],[146].
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