Deletion of VEGF-R1 also results in embryonic lethality, although in this case, the defect is an overabundance of blood vessels.
Cheah et al. (19) recently showed inhibitory neuron-specific Nav1.1 deletion in mice, using the Dlx1/2-I12b-Cre Dlx1/2-I12b-Cre Dlx1/2-I12b-Creich causedrivertaneouScn1afl/+ic seizures and premature lethality, although the phenotype of Scn1af/+, Dlx-Cre micaused milder than that of spontaneousGAT-Crepilepticnd more comparable with conseizurese heterozygous Scn1andnock-out mice.
In both studies, inactivation of both tardbp and tardbpl (the latter either by gene disruption by ZFNs or by knockdown) resulted in a severe phenotype and early lethality, although the phenotype of double heterozygotes has not been reported and might more closely resemble the ALS genotype (if due to haploinsufficiency) and perhaps the (partial) knockdown phenotype.
Conversely, mouse C2C12 skeletal myoblasts fail to differentiate following downregulation of Bin1 [ 19, 20], and Bin1 homozygous knock-out mice develop hypertrophic cardiomyopathy leading to perinatal lethality, although skeletal muscles do not exhibit any apparent abnormalities at this stage [ 21].
A null mutation in LRP-1, like APL-1, leads to arrest and lethality although at later larval stages [40].
Developmental heart defects are the most likely cause of embryonal lethality, although other contributory factors have not been ruled out [9].
Indeed we observed that a deletion in the rho-1 gene (rho-1 ok2418)) rho-1 ok2418lity, although rescausesperiments need to be performed to confirm that the rho-1 delethality responsible for this lethalthoughtype.
Indeed, they documented that simultaneous 5-FU addition or administration 2 days after cryotherapy resulted in synergistic lethality, although many cells survived these treatments.
The deletion of EGFR is also associated with embryonic or early postnatal lethality, although it is probably not related to cardiac effects [ 4].
Regarding the functionality of Ssa1-GFP, we have crossed it to ydj1Δ, which did not lead to lethality, although according to the SGD (http://www.yeastgenome.org/), the ssa1 mutant is synthetic lethal with ydj1Δ.
