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In this study, we exploited the green microalgae Chlamydomonas reinhardtii to produce dsRNA targeting the lethal shrimp yellow head virus (YHV).
Since ASDD has no effect on shrimp growth rate or survival, it has been considered a relatively minor problem in terms of economic loss in comparison to lethal shrimp viruses such as white spot syndrome virus (WSSV).
In this study, we employed a combinatorial approach to interfere multiple gene functions of white spot syndrome virus (WSSV), the most lethal shrimp virus, using a single-batch of dsRNA, so-called "multi-WSSV dsRNA".
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Go to El Azteca for the lethal margaritas and shrimp tacos.
These were not lethal to the shrimps as it is being controlled by the biofloc itself through higher diversity of phytoplankton and algae and also can compete with dominant number of heterotrophic bacteria.
Furthermore, a positive correlation between the lethality to brine shrimp and the corresponding oral lethal dose in mice of medicinal plants has been demonstrated by Parra et al. [ 37].
Searching among limpets and barnacles clinging to the rocks below the cliffs we found scarlet beadlet sea anemones, their tentacles sticky to the touch, adhering with their minute snares, which are lethal to any passing shrimp but which are unable to penetrate human skin.
Further the lethal concentration of brine shrimp can be correlated with the lethal dose in mice and was explained using medicinal plants earlier [ 63].
Alexandrium spp. species were found to have lethal effects on the brine shrimp at a density of 2000 cells/ml.
In fact, shrimp exposed to sub-lethal doses of virus induce a status of 'pseudo-vaccination' of the host, improving the resistance and survival of Parribacus japonicus to high infective doses of WSSV (Wu et al. 2002).
The results also imply that the sticky material on the surface of the body of the brine shrimp may have been an important lethal factor rather than the PSP toxins.
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