Sentence examples for lethal insertion from inspiring English sources

Revertants were initially identified based on eye color and reversion of the lethal insertion phenotype, then confirmed by PCR.

It would also simplify the stock-keeping of lethal insertions, since a Medea element tightly linked with the lethal insertion would constitute a type of balanced lethal.

The lethal insertion P{GawB}NP0434 that fails to complement mutant ELP3 alleles was obtained from the Kyoto Drosophila Genetic Resource Center, Japan.

Additionally, males with a new hemizygous X-linked lethal insertion would not survive and ones hemizygous for a new X-linked sterile insertion would be useless for generating a new stock.

Because the probability of misdiagnosing a lethal insertion rises if fewer progeny are analyzed, single-pair matings yielding less than 20 progeny were not used to make inferences about the lethality of the insertion (= 'uninformative single-pair mating' in Table 4) unless some progeny were EGFP negative.

The distribution of 280 homozygous lethal insertions on the linkage groups is shown in Figure 3.

Using this method, we obtained several hundred lethal insertions as well as insertions producing enhancer-trap phenotypes.

For 63 (28%) of the genes, we recovered only lethal insertions and the remaining 12 (5%) contained semi-lethal or sterile insertions.

In 14% of all lethal insertions, piggyBac had clearly jumped into the coding sequence of a gene.

The distribution of the remaining 280 lethal insertions in the genome is shown in Figure 3. *based on NCBI map viewer, build 2.1.

However, the majority of lethal insertions (61%; see Table 3) were located in introns, apparently disrupting transcription or splicing of the affected gene.