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Different from existing methods that require large amounts of synthetic lethal data, our method merely relies on synthetic lethality information from two single screens.
Administration of 15 mg/kg CpdA to non-immunized mice was also lethal (data not shown), confirming that high-dose CpdA was intrinsically toxic.
This finding indicates that Δapm1 and Δrho3 cells are synthetically lethal (data not shown), thereby suggesting that Rho3 regulates both secretion and Golgi/endosomes by influencing membrane trafficking in co-operation with Apm1.
Our approach is conceptually novel and different from existing approaches for synthetic lethal data analysis.
At 100-200 nM concentrations, Lat B was clearly lethal (data not shown).
As in S. cerevisiae, mutations in these genes are synthetic lethal (data not shown).
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The supplementation of the germfree orotic aciduria mouse model with uracil, uridine, deoxyuridine, cytidine, or deoxycytidine rendered the enzyme deficiency non-lethal (data not shown).
Lethal experimental data was transformed into square root of (x + 1) where needed, before analysis.
In our preliminary study, very young rats did not sustain the high doses of BTX-A administration within the lethal dose (data not shown).
Using the roughly uniform observed distribution of spectral slopes, we estimate the mean waiting time for solar flares producing a given biological dose of ionizing radiation on Mars and compare with lethal dose data for a wide range of terrestrial organisms.
We downloaded the synthetic lethal interaction data from BIOGRID ([6]; version 2.0.31).
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CEO of Professional Science Editing for Scientists @ prosciediting.com