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Nadal also made 12 backhand errors (9 ground strokes / 3 returns) as he was pushed both deep and wide in the ad court from Djokovic's lethal cross court forehand and backhand down the line.
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The MGMT protein can rapidly reverse alkylation at the O position of guanine, thereby averting the formation of lethal cross-links [ 20].
The first group (Additional file 5 table S5, S5.1-3) comprises lists of 'large seed genes' (we have used 'large seeds' as a shorthand for seeds with increased endosperm proliferation, even though in lethal crosses these seeds are only large while they are still alive).
The KK line (KK101925) targeting lola was early lethal when crossed to insc-Gal4 and was therefore crossed to wor-Gal4, ase-Gal80 that has a more restricted expression area.
Since DysE6/+ had wild-type wing veins, non-balancer F1 progeny from the DysE6/TM3 and P-lethal insertion cross were analyzed for the presence or absence of a mutant posterior crossvein which is altered in DysE6 homozygotes.
We next compared genes called up in fis1X2x with those up in the two lethal interploidy crosses.
These two lines were lethal when crossed to each other and formed the almond group of complementation.
The effectiveness of ilk, mys, parvin, and talin RNAi lines had been tested in Schnorrer et al. (2010) and found lethal when crossed to the mesodermal driver mef2-Gal4.
In this library, 314 lines were available for the 687 genes that caused visible brain phenotypes, and 235 of these (75%) are also lethal when crossed to insc-Gal4 (79 nonlethal lines).
Since all lines causing visible phenotypes in the pilot screen are lethal when crossed to UAS- Dicer-2; insc-Gal4 (data not shown), we chose to screen for lethality first and analyze only the brains of lethal lines by confocal microscopy.
Homozygous Klb-deficient (KO) mice in C57BL/6 background were semi-lethal, and crossed with Balb/c mice; male mice used were in mixed background.
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