Lethality is defined as the lack of homozygous animals upon genotyping (usually near wean); at least 28 animals must be genotyped prior to calling a strain lethal at 95% confidence.
Thus, the acute toxicity of ME is preliminarily evaluated as median lethal dose with 95% confidence (LD50 = 2.0 ± 0.2 g/kg) calculated according to the improved Bliss method.
Even the New York Times, which had fought in court alongside the ACLU to have it released, could only bring itself to editorialize mildly that the document offered "little confidence that the lethal action was taken with real care" and suggest that the rubber-stamp secret Foreign Intelligence Surveillance Court be involved in future kill orders.
CFU: Colony forming units; CHAB: Cysteine heart agar supplemented with 9% sheep blood; CI: Confidence interval; LD: Lethal dose; MLVA: Multi-locus variable number tandem repeat; PFGE: Pulsed field gel electrophoresis; SNP: Single nucleotide polymorphism.
Using samples from the posterior distribution and intercept parameters from the probit analysis, we estimated the 50%% lethal dose (LD50) and 95%% confidence intervals as well as the likelihoods of each strain being more or less potent than any other strain at the median lethality level.
The median lethal concentrations (LC50) and their 95% confidence limits were calculated.
The median lethal concentrations (LC50) and associated 95% confidence intervals (CIs) were calculated by Probit analysis (Probit analysis program, version 1.5.; WEST, Cheyenne, WY, USA).
Two days later, before its second vote of confidence, the government faced a lethal threat from Silvio Berlusconi, leader of the right-wing People of Freedom (PdL), to withdraw support.
lethal concentration with 50% lethality.
Thus, evaluating 20 single-pair matings is sufficient to exclude false-positive lethal lines with a very high level of confidence.
