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Following, a silane drop (Ceramic Primer, 3 M ESPE, St. Paul, MN, USA) was applied, let to dry for 30 s and air blasted.
The coupons were previously cleaned by immersion in a 70% ethanol solution, and then aseptically and individually washed with ultra-pure sterile water and let to dry overnight at room temperature (21°C).
The sample was then let to dry in air for 5 min. The emulsion was prepared by putting 300-500 μl of the aqueous suspension containing the nanoparticles (alumina of 50 nm nominal size, particle concentration 15%, Buehler, USA) into 5 ml of an insulating fluorocarbon solution (Fluorinert FC-77, 3M Company, USA) and ultra-sonicating the mixture for 20 s.
We applied the ink onto the gold wires by dipping the wire into the ink, let to dry, and thereafter placed into an in-situ TEM side entry holder [35] (Nanofactory Instruments).
Bacterial suspensions were added to the surface of the filter paper in the plastic vials and let to dry for 15 min. Flies starved for food and water for 7 h were anesthetised with CO2 and transferred to the vials per batches of 10 to 11 for a minimum of 30 flies per experiment.
After staining, the filters were let to dry and photographed using Canon PowerShot G6 camera.
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Let sit to dry.
Let stand to dry in a warm spot free of moisture.
Now, you have to let your nails to dry.
Let the finish to dry overnight.
Let sit somewhere to dry for about 12 hours.
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