In the standard method, 1X PBS-homogenized brainstem (at a final concentration of 2% or less) was incubated with 0.25-4 0.25-4Cl in a 10 μl voluMe for 1 hr at room temperature, followed by dilution of all samples to 0.25 M GdnHCl in 1X PBS and detection of the level of PrPSc remaining by ELISA.
The savagery was the culmination of generations of abuse and dysfunction, a dreadful violation that was far from inevitable but that had none the less been incubating for decades.
Sliced breast cancer tissues (thickness varied among samples, but was always less than 0.5 mm) were incubated for 1 h at 37°C with [I]TAZ (final concentration 1 nmol/l) in Krebs-Ringer phosphate buffer (pH 7.4), with 2% bovine albumin and 0.25% glucose [ 31, 32].
Aliquots of LC and SC poly P extracts (always less than 1.5 nmol) were incubated for 15 min at 37 °C with 60 mM Tris/HCl (pH 7.5), 6.0 mM MgCl2 and 3000 5000 units of purified rPPX1 in a final volume of 100 μl.
The TEER value in this culture appears to be strongly related to the amount of FCS in the medium from day 3 4 after seeding onwards, with cultures that were incubated with less FCS having higher TEERs.
When 14C hexabromobiphenyl and heptabromobiphenyl isomers were incubated in soil less than 0.2% of the 14C was volatilized.
The damage was less severe on the plants that were incubated at +4°C prior to freezing than on plants directly transferred to -15°C (Table 1).
Interestingly, the increase in membrane-associated u-PA was not observed when monocytes were incubated with the less aggressive breast-derived MCF-7 cancer cells (results not shown).
For experiments using peptide or ABT-737 alone, mitochondria were incubated with solvent (less than 0.5% DMSO), different concentrations of Bim, Bad, Noxa BH3 peptides, or ABT-737 for 1 h in 37°C water bath.
When PBMCs were incubated with killed trypanosomes, the effect was similar, albeit less drastic.
