Advantages with the new strategy are: (i) faster and simpler template preparation procedure (one-step); (ii) no need for exonuclease I treatment; and (iii) less problem with unspecific priming from loop structures and dimers.
Thus, we propose a similar situation where less efficient or unspecific binding of PhoP to regulatory motifs results in the loss of regulatory control of genes involved in cell-wall lipid synthesis resulting in the obvious phenotypic differences between H37Ra and H37Rv, and, additionally, as part of a compensatory mechanism, the over-expression of PhoP by H37Ra.
This measures ratios more reliably and is less affected by unspecific signals than the 'ratio of medians' (see also [ 41]).
Low level allergic sensitization may be unspecific and less related to clinical atopic disease compared to higher levels of sensitization [ 32].
Hence, inhibition of one single HDAC isozyme could be more effective and less toxic than the unspecific inhibition of several HDAC family members by creating a larger therapeutic window.
We did not want to miss potentially relevant risks of VAE treatment and consider it less problematic to overreport unspecific side effects than to miss a relevant major risk.
Classification of causing mechanisms using information provided by whole care episodes, instead of a single record, is less sensitive to missing and unspecific E-codes as record-wise inspection would postulate and therefore a more reasonable approach.
Constitutional symptoms, fever, and weight loss are unspecific and present in less than 40% of spinal TB cases.
Regional differences nearly disappeared and time trends were less marked when using combined specific and unspecific diagnoses of OB or UGIB, respectively.
Studies have shown that factors such as a gradual onset of symptoms, no motor dysfunction, unspecific and mild symptoms are less often recognised as representing stroke.
