Many public web-based sequence resources (e.g., NCBI GenBank and EMBL Nucleotide Sequence Database) have been developed to address the quality, redundancy and less-than-full-length nature of EST sequences [ 11, 12].
According to our model, N-terminally truncated PrP binds Dpl with less efficacy than full length PrP, even if over expressed.
Additionally, we show here SPR data indicating that truncated PrP binds to Dpl with ten folds less affinity than full length PrP (Figure 8 and Table 1).
This discrepancy in sensitivity of apical and basolateral membrane surfaces was also observed towards CROP-deleted TcdB (TcdB1 1852), although TcdB1 1852 was less potent than full length TcdB.
In contrast, over-expression of Rad60-SLD2Δ rescues the temperature sensitivity of rad60-1, but is less proficient than full length Rad60 in restoring resistance to HU and MMS, particularly at high doses.
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Physical mapping of the 5' and 3' ends of RC DNA and sequence analysis of CCC DNA revealed that CCC DNA synthesis requires the removal of the RT and an RNA oligomer from the 5' ends of minus and plus strand DNA, respectively, removal of sequences from the terminally redundant minus strand, completion of the less than full-length plus strand, and ligation of the ends.
Proteins having extensive internal repeat sequences are not well tolerated by Escherichia coli, hence the use of less than full-length versions here (MWrmfp-1 = 14KDa vs MWmfp-1 = 92KDa).
After single-linkage clustering, the MCL algorithm (Enright et al. 2002) was applied with I = 1.2 to each group using the lesser of hit-query bidirectional BLAST bitscores, normalized to self-hit bitscores, as edge weights but with hit-query length mismatches 30% set to zero to lessen the influence of less than full-length alignments on ORF clusters formation.
