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A less stringent method may therefore be needed to analyze microaray data.
Using a less stringent method with BLASTP (E-value ≤ 1E-05), these percentages were estimated to be 38.5% and 27.3%, respectively.
With the less stringent method, 67%and25%5% of P. rapae-induced genes in Rivera were only induced in this cultivar after 48 and 72 h, respectively.
We also analyzed some of these data at two standard deviations above empty spot mean signal intensity and found that this was a less stringent method of thresholding (data not shown).
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While the Food and Drug Administration recommends a sanitizing step like the chlorine treatment, it does not require it and some growers skip it or use less stringent methods.
However, we acknowledge that our results would not survive other – less stringent – methods to correct for multiple testing.
Based on the results obtained in the above analyses (comparing response versus status and "more stringent" versus "less stringent" methods), results for onset and durability were only reported for ACR50 and LDAS, as these measures were of comparable stringency and numbers of responders were high enough to enable meaningful interpretation of the results.
Only in extreme cases, when vital interests had to be defended against enemies undeterred by less-stringent methods, did the league engage in organized warfare.
The widespread nature of the risk is illustrated by the fact that just under half the respondents said they use less-stringent methods to protect data in development and testing environments.
In our experiments, primers were retrieved from the GETPrime database based on Ensembl release 50 with the most stringent parameters if available, or with the slightly less stringent design method 'DesignRelaxed'.
Paralog assignment suggested that three (RFC 36, 37, 40) of five RFC subunits are paralogous; i.e., originating from a common ancestor, whereas the result obtained by the less stringent BLASTP method suggested that all five subunits are mutually paralogous.
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