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These bioactive core of c-Src and c-Abl have less sequence similarity (∼45%) and good three dimensional structural resemblance (∼75%) whereas docked 4-amino-substituted compounds have a majority of hydrophobic atoms.
Observing less sequence similarity of the conserved hydrophobic amino acids and the good structural resemblance at the active core of c-Src and c-Abl kinases became focused to understand the role of hydrophobic interactions, binding affinity and the functional efficacy of ligands.
The results also indicated that the percentage of seed-specific genes among HS, MS and WS gene models both differed and were lower where less sequence similarity occurs.
Alternatively, it is possible that there are functional homologues with less sequence similarity to prototypical T4SS proteins in the B510 genome.
It is possible that either nuoG is shared with the two putative Complex I gene clusters or an alternative gene with less sequence similarity functions as nuoG.
Linked contigs with less sequence similarity were classified as either putative duplicates 85-955% similarity) or alternatively spliced transcripts (<85% sequence similarity), both of which were supported by comparing our classifications to existing gene models.
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It should be emphasized that not all true CNVs could be detected by qPCR, especially some low-copy duplications with less sequence similarities.
The other domains of Dmp53 show less obvious sequence similarity to Hp53, but appear conserved in function.
We next investigated the potential of the approach to assess functional conservation between gene pairs with less stringent sequence similarity.
It is comprised of three modules that solve the annotation process with progressively less-stringent sequence similarity requirements, combined with methods to optimize specificity.
Precise function can be reliably transferred only if sequence identity is at least 40% [ 11]; structure is significantly less conserved when sequence similarity is less than 50% [ 12].
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