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This probe hybridized more weakly than P1 and P2, which may be due to less sequence overlap with the targets, and/or its low (27%) G+C content compared to the full intergenic region (41%).
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The overall redundancy of the MGA was calculated to be less than 5.3%, thus a maximum of 138 of the 2,614 DNA fragments have a sequence overlap with another insert.
For species-specific AMP databases, AVP and HIPdb are found to contain less overlapping sequences.
The family tensions and action sequences overlap throughout the drama.
All of these sequences overlap with predicted CDSs.
These parameter choices will increase the time needed for the search to complete but will increase the likelihood of obtaining a hit, particularly in the case of the flank length choice, as shorter flanks are less likely to overlap sequences containing gaps between the sequences being aligned.
Thus, even though the number of excluded CDS was less than the number of FL-NAE, FL-cDNA sequences overlapping with the excluded CDS in TIGR4 might have been mapped as FL-AE.
In the future, there will be less overlap, less knowing the same stars everyone else knows.
Analyzed sequences overlapped those amplified in the qPCR assay.
Sequences overlapping with other URs were excluded from flanks.
If RefSeq mRNA sequences overlapped, only the longest was considered.
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CEO of Professional Science Editing for Scientists @ prosciediting.com