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Microfluidic flow cytometry generally permits higher sampling rate, less shear stress to cells, and less sample preparation steps than microscope-based cytometry.
Other methods have less sample preparation and use less expensive equipment and thus are available in more laboratories and have higher throughput.
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Solid-phase microextraction (SPME) is a solvent-less sample preparation method which combines sample preparation, isolation, concentration and enrichment into one step.
Thin-layer chromatography is a very popular technique for the assessment of TCMs in Chinese Pharmacopoeia, due to the advantages of lower cost, less rigorous sample preparation, higher throughput, and easier visualization (Sowa and Subbaiah 2004).
By comparison, the "in-cell western" (ICW) technique has high-throughput capacity and requires less extensive sample preparation.
Moreover, a method for labelling should be easy to handle with a less tedious sample preparation and it should be applicable for every kind of sample [52,74].
The arrays are generally considered easier to use with less complicated and less labor-intensive sample preparation than RNA-Seq.
It required less time for sample preparation and quantification compared to HPLC.
1H NMR is less sensitive but sample preparation is generally faster and analysis times shorter, resulting in markedly lower analysis costs.
H NMR is less sensitive but sample preparation is generally faster and analysis times shorter, resulting in markedly lower analysis costs.
The material composition has been investigated using high-performance liquid chromatography (HPLC) [ 28], Fourier transformed infrared spectroscopy (FTIR) [ 29, 30], and Raman spectroscopy [ 31, 32], a technique which requires less effort in sample preparation with respect to FTIR and allows one to assess collagen concentration, mineral-to-organic ratio, and cross-link typology.
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