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TATA less promoters depend on TBP participation in the pre-initiation complex by localizing it to the promoter through other DNA binding proteins.
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While the VP16 Inr sequence is identical to that of several murine TATA-less promoters, the VP16 Inr requires a TATA box for measurable activity.
Our studies provide insight into novel mechanisms of RNA Pol II transcription from multiple TSS-containing TATA-less promoters.
Second, the XCPE1 and XCPE2 sequences are mainly found in TATA-less promoters that often show multiple start sites.
TFIID dependence on Bdf1 for promoter recruitment is common at housekeeping genes that generally have TATA-less promoters.
We identified a new core promoter element XCPE2 that are found in multiple TSS-containing TATA-less promoters.
These are mainly housekeeping genes that tend to be SAGA-independent and have TATA-less promoters [42], [43].
Consistent with an increased transcriptional effect, we observe an elevated promoter occupancy by TBP in vivo in TATA containing and TATA-less promoters of differentially expressed genes.
Bdf1, like Rsc1, is a tandem bromodomain-containing protein that is important for recruitment and retention of TFIID at TATA-less promoters [32], [38], [41], [43].
More than 80% of mammalian protein-coding genes are driven by TATA-less promoters which often show multiple transcriptional start sites (TSSs).
The miR-22 promoter lacks TATA box and Initiator core promoter elements, but nevertheless directs a single major TSS which is unusual in TATA-less promoters.
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