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Optimized MA-CISH exploits discontinuous flow of DNA probe solution, enabling the reduction of the hybridization time from overnight down to 2 h and requiring 70% less probe solution, while achieving a better signal quality than the standard CISH technique.
Time-of-flight (TOF RBSS using a micro channel plate (MCP) further increases the sensitivity because of the increase in acceptance angle, which realizes the visualization of nanostructures with a beam spot diameter less than 10 nm with less probe damage.
Topically applied imaging agents have the advantage of using significantly less probe and avoiding blood flow and extravasation limitations (but not diffusion limitations) to transport.
Asymmetric PCR amplification can also mitigate probe hydrolysis because less primer extension on the probe binding strand causes less probe hydrolysis.
However, it should be noted that both these families were represented by two or less probe sets.
For the two abundant targets, 5S rRNA and 28S rRNA, we saw more signals with the Turtle Probes at equimolar amounts of probe, and approximately the same number of signals with an order of magnitude less probe.
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Local newspapers, many owned by conglomerates with business interests in the mainland, have become less probing.
Enough Said is gentler than Veep, but no less probing in its own way.
This one, without the emotional burden, was no less probing, but it was lighter - and a valuable watch for our decision-makers, I think.
Unfortunately, Cox's performance is less probing, portraying a feckless posh boy who grows up fast but not one who seems really terrified at the prospect of a firing squad.
This implies that microarray-measured differences in gene expression might be due to either differences in the frequencies of highly probe-complementary vs. less probe-complementary gene family members, or to the simultaneous up- or down-regulation of whole gene families.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com