If the latter is the case then comparison with a more recently acquired and less passaged gonococcal isolate should provide evidence of CREE-mediated deletion events.
Mouse embryonic fibroblast (MEF) cells were isolated from mouse embryos and were used at their early passages (less than passage 4).
And two Scots also navigated booking-less passages through their careers: Liverpool's Billy Liddell, whose mercurial displays during the 1950s helped win his team the nickname 'Liddellpool'; and Eddie Gray, a member of Leeds's rock-hard side of the 60s and 70s.
Is there a less realistic passage in the Bible?
She added, in a less noted passage, that giants on the court, including Justice Benjamin Cardozo, the second Jewish justice, had on occasion stumbled.
In a less publicized passage, it called on the Bush Administration to make "energy security a priority of our trade and foreign policy," and to encourage Middle Eastern countries to open up their energy sectors to foreign investment.
The Pope passed through the narrow door and down the precipitous steps to the manger in safety.In Israel on his way to Nazareth and Mount Zion Pope Paul enjoyed a less hectic passage.
To prepare conditioned medium of adipose-derived MSCs, 90% confluent MSCs (less than passage 5) were washed with PBS and then exposed in fresh co-culture medium for 24 h (Yang et al., 2013).
Supporters will be hoping for a less dramatic passage as the horse named after a Polish-born composer, Qatari-owned and Irish-ridden, looks to be a big winner for Germany in this year's "Derby of Derbies". .
The level of air pollution is inversely related to the extent of solar UVB that reaches earth surface, consequently, more pollutant areas, less UVB passage and as a result, lowers vitamin D cutaneous synthesis.
Isolated MEFs in early passages (less than 3 passages) were used for further experiment and cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 2 mmol/L L-glutamax, 0.1 mmol/L nonessential amino acids (NEAA), 100 units/mL penicillin and 100 μg/mL streptomycin.
