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Patient samples with higher percentage of trophozoites and late rings require less incubation time to reach maturation (unpublished data).
The catalytic activity at low temperature and maximum hydrolysis of the pectin in less incubation time indicate that PGase from T. frigidphilosprofundus would be a potential candidate for varied applications.
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In spite of the less sensitivity and longer incubation time for the observation of color changes, probe 1 could also provide an easy and simple detection method through naked eye.
Due to the present technical limitation of the used DLR clinostat device, ultra-short clinorotation experiments (less then 1 min incubation time) were not possible.
The bactericidal activity of this LFchimera was found to be drastically stronger than that of the constituent peptides, as was demonstrated by the need for lower dose, shorter incubation time and less ionic strength dependency.
In contrast, incubation time had less effect on the phytase activity of Yerong flour (Fig. 1).
An incubation time of less than 4 h did not change the amount of Aβ peptide detected in the supernatant, which is consistent with a previous report [62].
Preliminary adsorption kinetics showed that an incubation time of less than or equal 2 h was needed to reach equilibrium.
Both Massilia sp. and P. octavius are slowly growing and fastidious and therefore may be difficult to detect using the culture-dependent method, if the incubation time is less than 48 h and is not anaerobic.
This experiment was repeated using less chromatin and a shorter antibody incubation time, giving similar results but with lower overall enrichment (Additional file 1: Figure S3).
Although all incubation times could be used for HTS, the signals and signal-to-background ratios suggest an optimal incubation time of 120 min or less for the BODIPY FL vindoline (20 -based hPXR TR-FRET assay.
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