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Red-shifted acceptors could cure these drawbacks, but these are also likely to have less spectral overlap with CFP and thus less FRET.
When Variant 2 is stressed, the acceptor peak decreases and the donor peak increases, indicating less FRET and longer distance between the fluorophores in this condition.
Our intramolecular FRET experiments with Alexa350 and Alexa488 indicate less FRET between the donor-labeled carbohydrates and acceptor-labeled adjacent protein residues when the samples are stressed than in the original samples.
When acceptor molecules were bleached, less FRET interaction was observed, resulting in an increase in fluorescence.
After addition of CDCA the lifetimes increased to 0.950 ± 0.018 and 2.815 ± 0.033 in the same experiments, indicating that there is less FRET, likely corresponding to less ATP within the cell.
(C ) Using WT MutL (400 nM) mixed with the MutSΔC800 in this assay results in more binding at equilibrium than MutSΔC800 alone, while there is less FRET than for MutSΔC800 alone.
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At a Democratic caucus meeting last week, Senator Harry Reid of Nevada, the majority leader, was peppered with questions about the ruling from anxious senators, whom he told there should be less fretting about things they cannot control, an attendee said.
Like my daughters, I need to learn to do more applauding and less fretting.
Because these conditions are so specific, trustbusters fret less and less about the "co-ordinated effects" of mergers and more about the "unilateral effects"—the loss of competition between merging partners.The focus on market definition has nevertheless made it harder for trustbusters to stand in the way of some mergers.
If you spent less time fretting about your logo on a tool and just use what's there, we might get some work done.
Short of that, it seems to me that the best way to preserve the sanctity of American marriage is for us all to spend less time fretting about other people's marriages -- and more time improving our own.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com