This ETT2 gene cluster is highly homologous with that in meningitis-causing E. coli K1 strain EC10 (O7:K1), but contained less deletions and no insertions.
For the marker-less deletion of PF1950 a negative selection with 6-methylpurine was performed to induce a second recombination event which enabled the removal of the complete plasmid together with the resistance cassette (pop-out mechanism).
We have previously developed procedures for the creation of in-frame, marker-less deletion mutants in the pathogen Clostridium difficile based on the use of pyrE and codA genes as counter selection markers.
The delayed onset of epidermis and hair follicle defects seen in Pofut1/Tgfb3-Cre mutants may be due to less efficient deletion of Pofut1 than Rbpj by Tgfb3-Cre or remaining Notch activity from Notch receptors in the absence of O-fucose modification [9].
We noticed less Atp6ap2 deletion in all brain tissues analysed in Atp6ap2 Camicecompared0 mice compared with Atp6ap2 CamiceCre/0 mice (Supplementary Material, Fig. S2B).
Pawlak and Kini [ 141] reported a similar, though less extensive deletion in the Boiga dendrophila QC; thus it is clear that this sort of alternate splicing/post-translational modification is characteristic of snake venom QCs.
In a later investigation that included patients showing facial musculature, less homozygous deletions of the SMN1 gene (51% deletions, compared to the 100% earlier reported) in black South African patients were observed [ 13].
By using two distinct biosynthetic pathways, we demonstrate that DNA assembler can perform multiple tasks, including heterologous expression, introduction of single or multiple point mutations, scar-less gene deletion, generation of product derivatives, and creation of artificial gene clusters.
SHM introduces missense mutations and (less frequently) deletions or insertions into the V regions of Igs.
A marker-less gene deletion protocol based on a temperature sensitive knock-out plasmid T2-Ori was used to knock out the budC gene in B. licheniformis WX-02.
