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To address this issue, we first extract the DNA subsequences that interact with proteins since in general the DNA binding motifs are better conserved while the flanking sequences showed less conservation.
Collectively, our results confirmed our general hypothesis that NAD biosynthetic enzymes are evolutionary more conserved while NAD degrading enzymes show less conservation and potentially are still undergoing functional diversification.
Based on the phylogenetic conservation with known pre-miRNAs and miRNAs and their stability of folding stem-loop structures, 108 sequences [64 unique sequences with high conservation (E value≤e−4) and 44 with less conservation (E value>e−4)] were selected to predict conserved mature miRNAs in the Asian seabass.
The GTPase (G) domain is overall well conserved, particularly in the nucleotide binding loops, with less conservation seen in the exposed loops in between.
This revealed several evolutionarily conserved regions between bp −200 and + 100, but less conservation further upstream (Fig. 2A and data not shown).
Less conservation of gene synteny was noted in the zebrafish genome where only 4 conserved neighbouring genes were present in the MDA5 locus.
A little less conservation, a little more action please.
Moreover, temperate forests are afforded less conservation protection on average than are tropical forests.
Special attention is given to more common plant species, which have attracted far less conservation attention than rare species.
The same lemma, Lemma 2.4, will play a key role in the derivation of a less conservation delay-dependent condition than the general Jensen inequality in [38].
Moreover, the ompL1 gene showed more antigenic variation than lipL32 and lipL41 due to less conservation in secondary structural evolution within closely related species.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com