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However, this does not means that the proposed algorithm uses less bottleneck information, because prediction accuracy was also good for higher BC.
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Indeed, we obtain higher than observed levels of post-bottleneck MHC allelic diversity under a less severe bottleneck scenario suggesting that drift alone could maintain allelic diversity (100 individuals, Additional file 9).
Last year, the FDA approved 89% of applications for new drug uses, according to three experts writing at Health Affairs; that made the agency less a bottleneck than a rubber stamp.
The revolution in DNA sequencing technologies has enabled faster and cheaper data generation, to the point where data collection is becoming a less concerning bottleneck than data storage and analysis [1].
The significant positive values obtained in the neutrality tests may result from these types of recent and less pronounced bottleneck.
The second less pronounced bottleneck, which occurred approximately 50 - 100 generations ago, is most likely caused by breed formation.
Under a less severe bottleneck scenario of 100 individuals, simulated allelic diversity estimates far exceeded our observed values (Additional file 9).
However, it is also possible that the less severe bottleneck in the harsh condition, which decreased extinction probabilities (see previous section), acted to negate potential effects from low growth rates.
However, the temporal method never had power above 80% for less severe bottlenecks (N2 = 100) in the first two generations.
This building block was found to have less significant bottlenecks for skilled care at birth compared to other building blocks.
It has been proposed that the evolution of the virus is predominantly maintained by a reservoir in the tropics, where annual epidemics experience less severe bottlenecks, which increases the likelihood of local persistence [ 8].
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