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Therefore, this approach affords high sensitivity for telomerase activity detection and it can be regarded as an alternative to telomeric repeat amplification protocol assay, having the advantages of simplicity and less assay time.
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Fig. 1 Suppression of angiogenesis in vivo by novel compounds 10a, 12b, 14b, 10b, 10c and 14c in shell less CAM assay.
Alternatively, multiple demethylases could be functionally redundant in the removal of this mark, thus making deletion analysis a less ideal assay for identification of the enzyme responsible for erasing methylation at H2BK37.
This means that the results from our study may not be generalizable to labs that use a less sensitive assay.
Considering that VNT is a more specific and less sensitive assay, the actual positive rate could be >36%.
The removal of the UIM in the OTU-only construct rendered the protein less active (assay performed at a 14.5× higher enzyme concentration) and, importantly, nonspecific.
More recent and less cumbersome assays have detected and counted specific T cell responses at the single cell level using either ELISPOT assays of cytokine release [1] [4], or flow cytometry-based assays for intracellular cytokine production [5].
The use of less sensitive assays for HIV infection wrongly identified a deeply immunocompromised patient as an incident case.
NLV in children is often overlooked and/or under reported, particularly where less sensitive assays such as EM are being employed for diagnosis.
All the other less sensitive assays, namely the semiquantitative immunochromatographic method (PCT-Q, Brahms) and the luminescence immunoassay (PCT LIA, Brahms), should be used with caution and never for antibiotic stewardship [10, 38, 39].
More sensitive and less expensive assays for the early diagnosis of HCV are needed.
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