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However, cell sheets from 2D patterned surfaces have limited thickness, and harvesting the cell sheets for implantation is inconvenient and can lead to less alignment of myotubes.
In Fig. 2c, d, aggregates seeded in the middle and bottom of hydrogels show a less alignment of sprout as compared to the upper region of the hydrogel (Turturro et al. 2013).
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Fiducial-less alignment of the tilt-series was performed by feature tracking with TOM software (Nickell et al., 2005; Korinek et al., 2011) or patch tracking with IMOD software (Mastronarde, 1997), and tomograms were reconstructed using the weighted back projection algorithm.
But at higher speed, the mean fiber diameters increase and the scaffold has non-uniform fibers with less alignment because of fiber discontinuity (Fig. 5; Table 5).
The device has no critical parts such as narrow gaps or comb drivers, and it requires less accurate alignment of the magnetic field.
In the laboratory animals described here, a similar fibrous-looking area could not be identified, however, in histological sections a slightly less ordered alignment of the nuclei in the ONL could be observed in the streak region of all three animals examined, particularly prominent in M. unguiculatus (Fig. 4A F).
For this purpose, a multiple sequence alignment was generated based on a hierarchical method, i.e. similar sequences are aligned first, followed by alignment of less similar sequences (see methods).
RefSeq RNAs were aligned against the chimpanzee genome using blat; those with an alignment of less than 15% were discarded.
Acoustic waves were emitted normally into the PSi layers with uncertainty in alignment of less than 0.5°.
In contrast, stress distributions of the sample with low mechanical anisotropy were less affected by the alignment of the chain axis.
Extruded pellets are analogous to a composite material with 0.2 1.8 μm diameter 'fibres' of the liquid crystal being embedded in a less oriented matrix; greater alignment of the lamellae is observed in the 'fibres'fibres
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