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The CYP121 binding affinities (KD values) of the aminopyrazoles 5– 7 were determined by isothermal titration calorimetry (ITC) using direct titrations of ligand into CYP121, and their ligand efficiencies (LEs) were calculated from the KD values.
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Based on the phase space reconstruction of Duffing system output, the maximal LE was calculated to characterize the magnitude of the second harmonic.
The LE was calculated with decay correction.
Then, LE was calculated as a percentage by dividing the radioactivity of the labeled RBCs by the primary radioactivity with decay correction.
The LE was calculated for the overall ICU population and for each age group.
Life expectancy (LE) was calculated using age- and sex-specific cohort life tables [ 24].
Loading efficiency (LE) was calculated according to the following equation: (1) LE % = Analyzed weight of drug in nanoparticles Theoretical weight of drug loaded in the system × 100.
The LE was calculated using following equation [ 23]: (1) LE = [ Weight of the incorporated insulin weight of total insulin used for incorporation ] × 100.
Herein, LE was calculated using the binding efficiency index (BEI) defined as: BEI (LE) = pK i/MW [log unit/kDa] Furthermore, LipE was calculated as : LipE (LLE) = pK i – cLogP [log unit].
First, the illness-death model, like all life tables, assumes a stationary population with no changes in age-specific rates [ 51]; that is, for each of the two periods, LE is calculated under the assumption that the age-specific rates will remain constant until the last member of the cohort has reached the highest age.
For the highly and lowly potent partners of each cliff, LE and LipE were calculated.
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