The purpose of the study was to design a novel octa-arginine (R8) modified lipid emulsion (LE) system for the ocular delivery of the lipophilic drug disulfiram (DSF).
Production and purification of single constructs HIS-Cel5A-STREP HIS-Cel5A-STREPREP HIS-Cel5A-STREP and HIS-Bgl1-STREP HIS-Bgl1-STREP HIS-Bgl1-STREProteins that were prodisplayedh the potential system in E. cofi.
Since the LE system consists of a single system, methods C and E are similar (same thing for methods D and F).
In the LE system, i.e. the lessonae-esculentus system, LR frogs exclude the L genome during gametogenesis and make exclusively clonal R gametes (Fig 1a; hemiclonal reproduction, hybridogenesis).
Such a situation is currently observed in the Swiss LE system, where RR offspring used to die due to homozygosity of deleterious mutations [ 13], but now in many places survive and take over due to introductions of P. ridibundus [ 56].
In the LE system, females prefer LL to LR males [ 45- 47], but it is not known if females can and do distinguish between male genotypes in the all-hybrid populations.
In the LE system, LL males with scramble competition behaviour have more mating success than territorial LR males [ 53], but no data are available on genotype-specific mating success of LLR, LR and LRR males.
In the LE system, this asymmetry leads to an expected (Fig 1a; [ 17]) and observed (~60%, [ 16]) female bias, while in diploid RE populations, all hybrids are males (Fig 1b; [ 18]).
As to the diploid frogs, LR males make R gametes, like in the LE system, and LR females make both R and LR eggs, the latter giving rise to triploids upon fertilization by haploid sperm [ 24- 26].
