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Phages LES φ2 and LES φ4 have been previously isolated from the LES strain LES-B58 (James et al. 2012).
CHA, PT22 and 491 harbour copies of LES-prophage 1, LESGI-2 and LESGI-4 of the LES strain and a copy of an RGP29-insertion in the completely sequenced strain PACS2.
In this study, we investigate the ecological consequences of single and multiple phage carriage using two temperate phages isolated from the LES strain in experimental infections using an insect model system; larvae of the greater wax moth (Galleria mellonella).
Using an insect model of infection, we have investigated the role of two such phages, LES φ2 and LES φ4, in the success of the aggressive LES strain of P. aeruginosa in vivo.
Strains shared islands and prophages that have first been reported in the transmissible LES strain, but they were distinct in their PAGI-2/pKLC102-type PAGI-2/pKLC102-type PAGI-2/pKLC102-typehe extensislandse pool of thathonorecruiteudomonads.
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10.7554/eLife.01385.004 Figure 2. Rats of the SD strain helped trapped rats of the LE strain only if they were familiar with an LE individual.
The time to transit between DRL-LH 5 s and DRL 60 s varied widely between strains and within the LE strain.
Briefly, the eggs were collected from the livers of a laboratory population of out-bred Swiss mice infected with the LE strain of S. mansoni.
Swiss mice were individually infected subcutaneously with 100 cercariae/animal of the LE strain of Schistosoma mansoni (FIOCRUZ, Belo Horizonte, Brazil) in order to obtain the adult worms.
In contrast to SD rats with no exposure to the LE strain, most SD rats familiar with one LE individual became openers for LE strangers (8/12, 67%; χ2, p=0.03).
Schistosoma mansoni LE strain was maintained by routine passage through Biomphalaria glabrata snails and BALB/c mice 20-255 g) from the animal facilities of Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (FMRP - USP).
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