However, they failed to determine which factor specifically induces inflammation in LE lesions.
To determine whether the inflammatory lesions seen in the vicinity of SBCs after irradiation might also be present in established LE lesions, biopsies of 20 LE skin lesions were assessed for co-localisation of infiltrate and SBCs (Table 2).
For example, pDCs express high levels of L-selectin, and dermal endothelial cells in LE lesions express PNAd, a ligand for L-selectin.
Furthermore, Meller et al. [ 34] reported that UV light-induced injury leads to apoptosis, necrosis, and cytokine/chemokine production that results in an amplification cycle leading to cutaneous LE lesions.
This group of patients could not be distinguished from the other patients by any of the patient characteristics listed in Tables 1 and 2. In the patients who developed infiltrates, levels of T-cell and macrophage influx were in the same range as seen in 20 skin biopsies from patients with cutaneous LE lesions.
This group of patients with cutaneous LE lesions could not be distinguished from the patients who got UVB irradiation to the skin by any of the patient characteristics listed in Tables 1 and 2. Influx of T cells and macrophages was not limited to the dermal layer of the skin.
aPC = plasma coloration at 4d post-inoculation (log10 optical density at 480 nm)); T° = rectal body temperature (°C) at 4d post-inoculation; HEMA = hematocrit level at 4d post-inoculation; LES = lesion (scaled from 0 "no lesion" to 4 "most severe lesion") at 7d post-inoculation.
Abbreviations: d, Deiters' cell; op, outer pillar cell; ph, phalloidin; E-cad, E-cadherin; WT les., wildtype lesioned; Mut les., mutant lesioned; TM, tamoxifen.
Additionally, H&E-stained sections from biopsies taken before and after irradiation and biopsies of LE skin lesions were assessed for the presence of inflammatory lesions and co-localisation with SBCs.
Third, several reports have suggested that multiple UVB exposures induce macroscopic LE-like lesions [ 39, 40].
An additional disease component, lesion expansion (LE), was evaluated at Aurora NY as well.
