A ce site, on peut trouver beaucoup d'informations, y compris plusieurs rapports (et une bibliographie) sur les travaux de l'IRD (ORSTOM) dans le domain du SIDA.
Netrin Gs are alternatively spliced in their LE domain regions, but the binding region, the LN domain, is identical in all splice forms.
The long 3 4 loops of each LE domain contain short antiparallel β-strands and protrude similarly to leaves from the stalk.
Netrin G proteins share a common multi-domain architecture comprising a laminin N-terminal (LN) domain followed by three laminin epidermal growth factor-like (LE) domains and a C′ region containing a glycosylphosphatidylinositol anchor.
The crystallized B-S1 structures of unliganded P-LE domains (PDB code: 1G1Q) and of sLeX-liganded E-LE domains (PDB code: 1G1T), and the E-S2 structure of SGP-3-liganded P-LE domains (PDB code: 1G1S) were employed as initial structures.
Here a 45 ns simulation for E-LE domains was performed to further test the universality of S1' allostery from S1 in different selectin members.
We further correlated the allostery of Lec domain to S1' with the reorientation of EGF domain to extended states, as observed in the simulations of P- and E-LE domains.
Mutations within laminin γ2 are most frequently located within the N-terminal LE domains and the L4 module, whereas mutations in the C-terminal α-helical domain are rare [4].
Particularly, an effective binding unit is formed by LE domains and SGP-3 peptide, a 19 N-terminal sulfoglycopeptide of PSGL-1 composed of three tyrosine sulfate residues Y605, Y607, and Y610, and a sLex-modified glycan at T616 [5].
Moreover, the slight (Fig. 8C) or dramatic (Fig. 8F) shifting in the distribution was well correlated with the transient (Fig. 8A) or sharp (Fig. 8D) difference in the RMSD for P- or E-LE domains.
