LE diet resulted in reduced fat accumulation without compromising growth, and feeding LE feed restrictively had the largest effect (~ 2% unit) on fat accumulation.
All animals were returned to LE diet.
The changes in ling diet with depth seem reasonable, and appear likely to be a direct response to changes in prey availability.
Isolated islets from healthy adult P. obesus on LE diet (Harlan; Jerusalem, Israel) were handpicked, as described [37], and incubated in RPMi 1640 supplemented with 1% penicillin/streptomycin, 10% newborn calf serum and 5 mM glucose (Gibco-BRL).
For these two traits, the QTL that showed an interaction with diet explained a larger proportion of variance for the LE diet than for HE diet.
The two other QTL (QTL62 and QTL105), which were detected for YOLKIND, accounted for a larger proportion of variance for the LE diet than for the HE diet.
The QTL that showed an interaction with diet were located on chromosomes 3, 9, 10, 12, 13 and 19, and nine of these 10 QTL had a greater effect for the LE diet than for the HE diet (Table 5).
Analyses of the four subsets estimated the effect of each SNP for each of the four conditions investigated (HE diet, LE diet, egg collections at 50 and 70 weeks of age).
For each trait, the GWAS analysis was repeated separately, as above, for the four datasets for each condition (egg collections at 50 and 70 weeks of age, and HE and LE diets) in order to estimate the effect of the top SNP for each QTL in the two environments, α1 and α2, i.e. egg collection at 50 vs. 70 weeks of age and HE vs. LE diet.
Among the QTL that showed an interaction with diet, QTL4 for ESSTIF and QTL102 for ESS accounted for a larger proportion of variance for the LE diet than for the HE diet, and QTL11 and QTL117 for ESS accounted for a larger proportion of variance for the HE diet than for the LE diet.
