Secondly a structured model reference adaptive control law was designed for the aeroelastic system with both TE and LE control surfaces.
Before walking with EMG-triggered transcutaneous NMES, the subjects were trained to activate paretic MG muscle for push-off using biofeedback of the LE control signal in order to trigger the NMES device.
For TC, there was a less significant relationship between REs and LEs in control subjects and diabetic patients with "none" and "severe" neuropathy.
In addition, the distal boundary of pigmentation in the control and Le-cre; Wls fl/fl eyes differs.
To test this idea, we counted the number of RPE and retinal cells in E11.5 control and Le-cre; Wls fl/fl mutant mice using Mitf and Pax6 for labeling.
When we examined the immunoreactivity for Raldh1 (Aldh1a1), Raldh3 (andh1a3) and Pax2 in dorsoventral sections of control and Le-cre; Wls fl/fl mice, we observed a very similar distribution (supplementary material Fig. S5), even though the altered shape of the retinal rim in Le-cre; Wls fl/fl mice gave the impression of higher expression ventrally.
To test this, we labeled E10.5 control and Le-cre; Wls fl/fl eye sections for the proliferation marker Ki67 (supplementary material Fig. S1), mapped positive cells from representative sections (n=11 for each genotype) onto a schematic of eye sections (Fig. 6D) and quantified Ki67+ cells in each of ten sectors that represented different optic cup regions (Fig. 6E,F).
To determine whether the active, phosphorylated form of myosin II that is normally found in the optic cup hinge (Eiraku et al., 2011) is modulated in response to the ectodermal Wnt signals, we labeled control and Le-cre; Wls fl/fl eye cryosections with anti-phospho-myosin antibodies.
Statistical analysis for the comparison of the ED50 and the maximal relaxation between the control and LE-treated groups in the acetylcholine-induced relaxation was performed using the Kruskal-Wallis test followed by Dunn's multiple comparison test or the Mann-Whitney test.
This is similar to the range of reduction seen by Mehrotra and McKim (2006) in the LE mutant c(2 M (44.8% of okra control) and the TF mutant c(3 G (15–21.3% of okra control) in the absence of repair.
