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At the end of the incubation period, the evaluation of lens opacification, MDA level, and the activities of SOD, CAT, GPx, and AR in lens were performed.
In patients with significant lens opacity, standard phacoemulsification and aspiration, and implantation of an intraocular lens were performed prior to DSAEK using the phaco-chop technique.
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Characterization of the lens was performed to determine its resonant frequency, index of refraction, focus spot size, and optimal focusing length (for proper sample location).
Anterior chamber angle was first evaluated statically, and then dynamic examination with the lens was performed.
HPLC analysis for sugar alcohol in lens was performed with this filtrate after being benzoylated [ 26].
Explantation and implantation of a scleral-fixed lens was performed (figures 3 and 4).
Right eye phaco-emulsification with implantation of posterior chamber intraocular lens was performed in 2008 with good visual recovery.
Gonioscopy (Volk's 1-mirror non-flanged lens) was performed if the IOP was ≥20 mmHg, or VCDR ≥0.6, or VCDR asymmetry ≥0.2, or VH grades 0, 1, 2. Central corneal thickness was not assessed.
Control: Age, TCCT, PCCT Dilated fundoscopy with slit lamp biomicroscopy and 78 D lens was performed by a specialist registrar (RW) to confirm the grading of CSMO according to the Early Treatment Diabetic Retinopathy Study extension of the modified Airlie House classification [ 11].
An axial depth scan in a lateral direction over the central region of the lens was performed to achieve two-dimensional spatially resolved determinations of the storage modulus and the loss modulus.
Stereophotomicroscopic analysis of dissected intact human lenses was performed on postmortem specimens obtained from enucleated eyes procured through national tissue bank resources (National Disease Registry Interchange, Philadelphia PA Floridada Lion's Eye Bank, Miami, FL).
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