With the fundus imaging and dynamic vessel analysis (DVA) results from Fig. 9 and Fig. 10, it can be resumed that the substitution of all system lenses for standard lenses was sufficient in terms of optical performance for imaging the major retinal vessels and performing DVA.
Because the technology is new, you'll pay more for these lenses than standard D.S.L.R. lenses.
A 3x zoom 18-55mm f/3.5-5.6G ED II AF-S DX Zoom-Nikkor lens comes standard, but other lenses can be popped on.
Images were acquired with a Leica TCS SP2 confocal system (Leica Microsystems) using an oil-immersion 63× objective lens and standard lasers and filters for FITC, Cy3 and DAPI (two-photon) fluorescence.
In patients with significant lens opacity, standard phacoemulsification and aspiration, and implantation of an intraocular lens were performed prior to DSAEK using the phaco-chop technique.
Image acquisition was performed using a digital inverted fluorescence microscope EVOS-xl microscope (Life Technologies, Carlsbad, CA, USA), using a 4×, 10×, 20× or 40× lens, and standard light microscope.
For images of fixed cells, cells on glass coverslips were mounted using DPX (Sigma) and confocal images were collected using a Leica SP5 confocal microscope equipped with a ×100 [NA (numerical aperture) 1.45] oil immersion lens using standard filter sets and laser lines for Alexa488, Cy3 and DAPI.
VF testing was performed with optical correction by contact lenses or by trial lenses by standard automated perimetry (SAP) using the Humphrey Visual Field Analyzer with the 30-2 Swedinteractivethresholdshold algorithm standard (Carl Zeiss Meditec, Inc. Jena, Germany).
Manufacturers provide objective lenses with standard magnifications usually ranging from 2 to 100×.
In a statement, Heather Carpenter, a Reuters spokeswoman, said: "The use of long lenses is standard photo journalism practice and many news organizations, including Reuters and The New York Times, use them as a matter of routine.
