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One of the most interesting techniques for lens preparation is in situ polymerization of multi-functional acrylic monomers, because a variety of polymerization methods and systems are possible.
One of the most interesting techniques for lens preparation is bulk polymerization of multi-functional acrylic-based monomers, because a variety of polymerization methods and systems are achievable.
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Retina-lens preparations were post-fixed in cold 4% paraformaldehyde solution for 4 hours at 4°C.
The resulted retina-lens preparations were post-fixed in cold 4% paraformaldehyde solution for 4 hours at 4°C.
Retina-lens preparations were obtained by first making a small cut at the edge of sclera toward the posterior pole.
After rinse in PBS, the retina-lens preparations were incubated with Alexa fluor 488-conjugate PNA (Invitrogen, Carlsbad, CA).
For PNA staining, retina-lens preparations were incubated with FITC-conjugated (Vector, Burlingame, CA) or Alexa fluor 488-conjugate (Invitrogen) PNA.
The analysis was carried out with cDNA prepared from independent pools of day E15.5 wild-type and dnBrg1 total lens RNA preparations as described in Methods.
The lens cell membrane preparations maintained the native double membrane architecture of the junctional microdomains.
Next, confocal images taken with a × 63 magnification lens on surface preparations of the lower basal turn (4.5 5.5 mm from apex) were used to count condensed OHC nuclei identified as apoptotic.
The method is appropriate for the preparation of 10 1000 spherical lenses in a glass capillary with a diameter of 0.1 1 mm.
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