This liquid has a refractive index of 1.55, hence we need an objective lens for oil immersion.
Images were captured with 60X lens under oil and analyzed using the Slidebook 5 software.
These cells were observed using a CLSM system (LSM510 META, Axioplan2 Imaging; Carl Zeiss) with a Plan-Apochromat lens (633 1.4 oil differential interference contrast; optical slices of 1 mm).
The two beams are spatially superimposed by dichroic mirrors and focused by the objective lens (100x oil immersion, NA = 1.4, Leica, Wetzlar, Germany).
All cytospin preparations of PBMCs were first examined under a conventional epifluorescence microscope (Leica) using 40 x objective lens with oil immersion and were further analyzed by confocal (Leica SP) microscopy.
In the four-channel Stokes-polarimeter imaging configuration, an objective lens (UPlanFLN40X/N.A. 1.3 oil, Olympus Corp., Japan) and a long-working distance 0.5 N.A. condenser provide excitation and SHG signal collection, respectively.
Spine densities on the dendrites of CA1 pyramidal neurons were observed under a Nikon Eclipse E400 microscope with a ×100 objective lens with oil immersion by an investigator blind to the treatment group.
Images were acquired on a Zeiss 510 Meta confocal microscope with a 63× oil lens and 40× lens, then analyzed using ImageJ Wayne Rasbandd, NIH).
Our optical device is composed of a tunable water prism and oil lens driven by electrowetting.
Suspensions stained with 0.02% Janus Green B were tested by oil lens of microscope to verify the quality of mitochondria.
