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A water immersion 63X lens objective (63x/1.20W Corr, apo) was used to account for the large working distance introduced by the montage.
The single lens collimator and double lens objective comprise an infinite conjugate optical imaging configuration [ 33].
For time lapse imaging, we used a Zeiss Axiovert 200 M microscope equipped with a ×20 lens objective and an Axiocam camera (Additional file 2) or a Leica SPE confocal microscope using a ×40 lens objective (Additional file 3).
The stained sections were visualized with a Nikon Eclipse E400 light microscope (Nikon) using a 40× lens objective.
The stained cells were visualized under a fluorescence microscope (Nikon Eclipse E600; Nikon Instruments) at a × 60 lens objective.
All images were captured using a 10× lens objective with MAG Biosystems software 7.5 (Photometrics, Tucson, AZ, USA).
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The JSM-7600F operates with the use of a T-FE electron gun and sem-in-lens objective lens in its electron optics system and a robust structure is able to operate in a broad range of installation environments.
The optical components such as scan mirrors, scan lenses, objective and GRIN lens system are compatible with this new excitation source.
Gissibl, T., Thiele, S., Herkommer, A. & Giessen, H. Two-photon direct laser writing of ultracompact multi-lens objectives.
The three front lenses of the objective lens are shown in Fig. 17.
In the sample arm, the combination of collimator, scan-lens and ocular objective lens provided ~16 μm lateral resolution on the surface of retina.
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