However, neither endogenous α3 nor α8 connexin proteins were reduced in P1 γB S11R/S11R) mutant lenses (data not shown).
As controls, expression of B2M, Hprt and Sdha was found virtually unchanged in total RNA samples prepared from wild type and Pax6 heterozygous lenses (data not shown).
Cells within each compartment traveled along similar trajectories (Fig. 7N, supplementary material Movie 8), with one exception: during pinwheel movement 12-144 hpf), the posteriormost lens cells stalled or moved anteriorly, initiating posterior lens compaction (data not shown).
Although the ZEMAX lens data is not available for the Nikon objective lens, we model the longitudinal magnification of the objective using a simple lens with the same focal length, 5 mm, and then, assuming no chromatic aberration in the objective, remove the chromatic shift associated with that simple lens to estimate the chromatic focal shift of the total lens system.
Age-associated changes were not observed in skeletal muscle, ocular lens, or brain (data not shown).
The Brg1 flox/+ Le-Cre mice appeared normal in terms of lens gross morphology (data not shown).
Immunofluorescence analysis via FLAG antibody confirmed transgene expression in differentiating primary lens fibers cells (data not shown).
It is noteworthy that the S-LAPs share extensive sequence homology (>40% at the amino acid level) to the bovine lens leucine aminopeptidase (data not shown).
Protein blot analyses of the proteins obtained from an ARC lens showed that most of the UPR proteins are degraded but Ero1-L β was still detectable, and its level remained for a long time in the lens fiber cells (data not shown).
Similar expression patterns of the above factors were observed in the mouse lens epithelial cells, αTN4-1 (data not shown).
