Measure 2 was designed as a more lenient sensitivity analysis to measure 1, which is a standard definition of persistence [ 12].
The more lenient post hoc analysis added no cases to these estimates.
The more lenient post hoc analysis added only 1 case to study 1 and 6 cases to study 2 and was therefore not further taken into consideration.
In this context, the smaller the required amount, the more lenient the trial sequential analysis, thus the more lenient the criteria for significance.
Overall, the above comparisons indicate that, even when using very lenient criteria for homology and analysis, over half of Cbr-GLD-1-associated mRNAs were not detected as Ce-GLD-1 targets in two independent studies.
The factor analysis results in five factors: Lenient sickness attitudes, Low intrinsic motivation, Lenient towards cheating, Being stressed and High homeload.
The slightly more lenient threshold for significance in the masked analysis reflects the smaller search volume (number of voxel clusters) tested.
To confirm that our results were not a product of our rather lenient LD threshold, we ran a secondary analysis where one SNP from each pair of SNPs with an r > 0.2 was randomly deleted.
At this stage, since the intention is to obtain a shortlist for further analysis in the pipeline, a lenient cut-off of 50th percentile was chosen so as to avoid any false negatives.
Using a lenient twofold or greater ΔRFC cutoff, this analysis defined two subsets of differentially regulated genes: those with higher overall expression in WT than in ArtΔ/Δ-positive (ΔRFC in Figure 8a) and those with lower overall expression in WT than in ArtΔ/Δ (negative ΔRFC in Figure 8a).
