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For each condition, 20 cells were analyzed and mitochondrial lengths were measured using Image J software.
Local bubble characteristics, including gas holdups, bubble rise velocities, and chord lengths, were measured using a monofibre optical probe manufactured to withstand elevated pressures.
After dissection of the tibiae from the surrounding soft tissues, their lengths were measured using digital calipers to be the following: LC young: 17.3 ± 0.4 mm, LC adult: 18.4 ± 0.3 mm Sost KO young: 17.7 ± 0.3 mm and Sost KO adult: 18.4 ± 0.3 mm.
Root and shoot lengths were measured using a scale.
Cell lengths were measured using Image J software.
Seedling plates were photographed and hypocotyl lengths were measured using ImageJ software.
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The corresponding lengths was measured using the 2D/3D skeleton PlugIn [49] for the NIH ImageJ software [50].
All crabs were counted and their length were measured using a vernier caliper.
The phase and period length were measured using Actimetrics software for LumiCycle with sin fitting as previously described36.
Both, increment of the primary root and lateral roots length were measured using JMicroVision 1.2.7 software.
The final concentration and distribution of length were measured using the Experion DNA 1K Analysis kit (Bio-Rad, Hercules, CA) on Experion Automated Electrophoresis Station (Bio-Rad).
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