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Second, the effects of different cluster sampling designs on the precision of mean lengths were estimated using the bootstrap method.
PCR fragments were resolved on 3% agarose gels and their lengths were estimated using Quality One software (Biorad) against a 50 bp DNA ladder (Invitrogen).
The branch lengths were estimated using the proportional model [ 45].
Maximum likelihood and variance-covariance matrix of the branch lengths were estimated using the Estbranches program.
The branch lengths were estimated using the one-ratio codon model (M0) from PAML [ 11].
Branch lengths were estimated using Tree Puzzle 5.1 under the WAG + Γ 8) model.
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Their lengths are estimated using the formulas (here and lower |w| is the length of a symbolic sequence w, i.e. |P| is the length of sequence P which is a common ancestor for cores of compared sequences): L 1 = P ∙ r ∙ 1 - c ∕ 2 ; R 1 = P ∙ r ∙ 1 + c ∕ 2 ; L 2 = P ∙ r ∙ 1 + c ∕ 2 ; R 2 = P ∙ r ∙ 1 - c ∕ 2. Generation of cores is described in subsection 2.1.3.
Genetic parameters for calving ease, stillbirth and gestation length were estimated using the restricted maximum likelihood method, considering different models i.e. sire (−maternal grandsire), animal, univariate and bivariate models.
The related crack length is estimated using an exhaustion method.
The filter length is estimated using an equiripple low-pass implementation and should be applied to both real and imaginary components of the baseband signal.
At harvest, the two root systems of each plant were separated; scanned using a root scanner (EPSON LA 2400) and their length was estimated using the Win-Rhizo software (Regent Instruments, Canada).
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