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Calculation of these scores would reflect the differences in two alignments of a pair of segments in terms of the substitution of PB at an alignment position as well as the lengths of the alignment.
The representative lengths of the alignment for trnH-psbA and trnS- trnG were 456 bp and 784 bp, respectively.
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The maximum lengths of the alignments (http://spiro.mmg.uci.edu/data) were set by the shortest available sequence.
In this formula, the identity t i of the true contigs i is calculated as the number of aligned bases over the length of the alignment.
Sequence identity was multiplied by the length of the alignment relative to the total protein length (≤1), which penalizes non-aligned regions.
Specifically, the standard Pearson correlation coefficient was calculated for each aligned pair of column vectors and summed over the length of the alignment to provide a raw score.
We call N the total length of the alignment covered by human and mouse ESEs, i.e., the number of aligned nucleotides labelled as H and M in Figure 8.
Other studies of brown bear have estimated various time to the most recent common ancestor (tMRCA) for other clades, in particular influenced by the length of the alignment (single mtDNA genes versus whole mitogenomes) and the method of calibration (e.g. tip-dating, fossil calibration 1,13,28.
Static ground water table was not encountered in any of the trial pits dug for the collection of soil samples throughout the length of the alignment.
Nucleotide similarity greater than 85% all over the complete length of the alignment was required to identify orthologous candidates (Supplementary Figure 1).
The scores were normalized by the length of the alignment.
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