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We employed various parameters of Bowtie and different lengths of read three to obtain the optimal mapping yield (Additional file 1: Table S2).
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Sequencing technologies vary in the number and lengths of reads they can produce.
Megabases of coverage was calculated based on the numbers and lengths of reads that were successfully mapped to the reference.
To obtain uniform lengths of reads, the 101 nt were trimmed from the low-quality (right) end of each read to only 76 nt before mapping.
In practice, because groups may have different number of reads, and lengths of reads may be different, this study uses a normalized frequency which is based on the total number of l-mers in each group.
The length of read in Grimmond data is 35 bp.
Trimming was performed using the fastx_trimmer with quality filtering by flagging –t for minimum quality threshold and -l for minimum length of read to be retained after trimming.
An alternative estimation of the length of identified repetitive DNA was performed using the number and total length of reads matching repeat sequences, identified above.
As the length of reads that got no hit are not dramatically smaller, we believe this indicates potential discovery of novel coding regions in camel.
Due to the shorter length of reads produced for the HI clone, some regions, which exhibited high genetic variation, had little or no alignments.
The read was only excluded if the length of reads was 17 bases after the trimming.
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