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We next sought to identify peptide substrates that would allow the determination of the Michaelis constant (Km), and also allow for varying sequence and length to probe substrate specificity.
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We also compared the expression levels of Thellungiella and Arabidopsis Na+-transport system genes by using full-length cDNAs to probe Northern blots under equal conditions of hybridization and detection.
We used all identified exons that were longer than 200 bp (average size 332 bp) in length to design tiling probes for the capture arrays.
Universal arrays represent a possible solution for re-sequencing of highly variable genes but increasing the probe length to above 10 nt increases the number of probes required above and beyond the capacity of a standard array.
The compounds are prepared with varying degrees of linker length in order to probe the structural requirements for optimal in vitro anti-tumour activity.
Given the pump rate and tubing length from pump to probe, the lag time for the drug to reach the probe was approximately 15 min; the effects of the drug were apparent within an 15 additional minutes, the lag time from the probe to the collection vials.
Factors considered include maximizing probe uniqueness in the genome to minimize cross-hybridization, and altering probe length to conform to a needed range of melting temperature.
The best strategy allowed probe length to vary within 32 40 bp to equalize hybridization free energy.
It was found that the thermal probe technique had potential to be a valuable tool in helping measure, manage and reduce the energy demand of buildings but that traditional solutions for probe length to radius ratios need revision before reliable results can be consistently achieved.
However, because our analysis could be extended to a multivariate format, we were able to test directly among the related variables Tm, GC content, and probe length to arrive at the predictor variable(s) with the largest effect(s).
We predict that these methods can be further generalized, with some modification due to differences in chemistry and probe length, to any hybridization array platform.
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CEO of Professional Science Editing for Scientists @ prosciediting.com