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In vivo 2P images were processed using Matlab (synaptic remodelling), Image J (length quantification, file conversions), Adobe Photoshop (section alignment, brightness and contrast, image rotation) or Neurolucida (axonal arbour tracing) as required.
(A – D ) Root growth and length quantification of Col-0 and pi4kß1/2 seedlings under high auxin conditions.
Telomere mean length quantification was carried out with an adaptation of the Q-PCR methods described by Cawthon [ 32, 33].
Examples of confocal images of PC-12 cells expressing indicated constructs used for neurite length quantification (left).
Relative telomere length quantification was determined by quantitative polymerase chain reaction (PCR) as described in detail, based on the method reported by Cawthon.
Most of the applications of high throughout telomere length quantification methods are derived from the association between telomere shortening and aging.
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The built-in measure function of LSM image browser (Zeiss) was used for the body-length quantification.
Sholl analysis calculated the number of dendrite intersections and dendritic lengths, Sholl quantification was conducted with an initial 15-μm somal radius and 20 μm concentric radial steps.
(d) Traces of limb length profiles and quantification of whole-limb extension during the support phase for controlled and non-controlled conditions.
was used for root length and biomass quantification.
Mean strut length difference provides quantification of the error related to the stent strut length as a relative percentage of their initial length.
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