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In six of the seven most active clones a wild-type serine at position 83 had been replaced by threonine, a substitution known to alter the substrate chain length preference of Lipolase® variants.
The FA chain length preference of rNa-FAR-1 was tested by displacement of the fluorescent ligand DAUDA as described [ 13].
Among the tested TAG hydrolysis reactions, Tll displayed the highest activity toward tripalmitin (C16 TAG), which indicates the fatty acyl chain length preference of Tll being C16 over C14 and C12.
As to whether there is a gene length preference for deaminases, for AID* and sA3G* datasets, we find no particular bias for mutations to occur at short or long RNAP II mRNA genes (see Author response image 1 below).
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Most epidermal fatty acyl residues in ceramides are ≥C28 chain lenth and skin expresses five different ceramide synthase genes with different fatty acyl-CoA chain length preferences; however, it is currently not known which of the Lass gene products catalyzes the ≥C28 acylation of ceramide [37].
Among other characteristics, these classes differ in their expression profiles and branch chain length preferences.
Global comparisons of the BDL hrPKSs and nrPKSs and separate alignments of their KS domains fail to cluster these enzymes according to the number of the extension cycles they are expected to conduct, nor do the sequences of the nrPKS SAT domains provide immediate clues for their substrate length preferences.
Now, it is possible to quantitatively test the predictions of our model for the dependence of L eq ∗ on protein charge and salt concentration through similar competition assays in which NA length preferences are observed for proteins with charge altered by mutagenesis under different ionic strengths.
If you always go to the same stylist, it's especially important to make them aware of your length preferences, so they'll remember the next time you come in for a cut.
Our analyses revealed a mismatched motif in the basal stem region, a preference for maintaining or improving base pairing throughout the remainder of the stem, and a narrow stem-length preference of 35 ± 1 base pairs.
The ADOs from Synechocystis sp. PCC 6803 and Prochlorococcus marinus MIT9313, and the corresponding variant A134F displayed less divergence, although variation between chain-length preferences could be observed.
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