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Primary neurite number and total neurite length per cell decreased on micropatterned PLGA films compared with the controls.
This strategy promoted guided neurite extension from PC12 cells of up to 10 μm total neurite length per cell in 2 days.
Total neurite length per cell was obtained by addition all neurite length from each cell.
Cell numbers were counted for each field by using propidium iodide (Molecular Probes) staining to reveal nuclei, and the mean ZO-1 length per cell was calculated.
To determine the maturation of neurons differentiated from transplanted SENAs, the longest neurite length per cell was measured using the Neurolucida software (MicroBrightField Europe).
To quantify the average ZO-1 length per cell, four fields were randomly selected from each coverslip, and the total length of ZO-1 at cell junctions in each field was outlined manually, followed by measurement using Image J software (National Institutes of Health).
Similar(30)
Besides their characteristic beating cilia (6 μm in length, 100 250 per cell), they also protrude microvilli (1.8 × 0.1 μm) into the lumen (Hansell and Moretti 1969).
Most of these methods provide mean telomere length values per cell or per sample and very few of them are able to measure the abundance of short telomeres, which are the ones indicative of telomere dysfunction.
This appears to be the predominant dimer interface within full-length proteins, and disruption of these interactions alters the mobility of full-length PER proteins in the cell.
(F) Average length of segments per cell.
(G) The total length of branches per cell.
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