Sentence examples for length of transcript from inspiring English sources
According to cDNA sequencing results, three transcripts were detected: a 635 bp length of transcript (1/20), a 513 bp length of transcript (1/20), and the majority 537 bp length of transcript (18/20).
The maximum length of transcript present was also considerably greater in the M' group than the S' group.
As reported in the previous study [ 4], the length of transcript sequences is crucial in determining the efficiency of BLAST searches.
FPKM values for the transcripts were determined using the formula, FPKM = (Number of reads Mapped × 10) / (Length of Transcript × Total Number of Reads).
The program produces two separate output files: (i) FASTA protein sequence and (ii) information on translation statistics including the length of the 5' untranslated region (5'-UTR), potential ORF length, potential ORF ratio (length of potential ORF /(length of transcript), 5'-UTR ratio (length of 5'-UTR)/(length of transcript), and protein length.
Then, the coverage (number of matches/full length of transcript sequence) of each alignment was calculated, and the low-quality alignments with coverage of less than 70% were removed.
ExN50 is the length of a transcript while the total length of transcripts shorter that it reached 50% of total length of all transcripts in this dataset.
By analyzing the length of transcripts and unigenes, we found that the numbers of unigenes of lengths of 200 500 bp, 0.5 1 kb, 1 2 kb or greater than 2 kb were 37,645, 12,190, 8714 and 3703, respectively.
For this analysis, we considered only those models where the sequence length of transcript-derived model differed by greater than 10% (plus or minus) from the initial Myc-BestModel.
Fragmentation of RNA and/or cDNA has been used previously [6] to achieve more even sequence coverage throughout the length of transcripts.
RNA and cDNA fragmentation are frequently used to improve evenness of coverage across the length of transcripts so splice junctions and SNPs can be more efficiently detected.
