Exact(1)
The length of the replication signals and the distances between origins were measured in micrometers and converted to kilo bases according to a constant and sequence-independent stretching factor (1 μm = 2 Kb), as previously reported (Herrick & Bensimon, 2009).
Similar(59)
(D ) Kinetic trace for strand displacement DNA synthesis using the fluorescence-based assay shows the increase in lag time when the length of the dsDNA in the replication fork substrate is increased.
This time point is relatively early in DENV infection, and was chosen to allow for sampling of the transcriptome while the virus was actively replicating: the length of one DENV replication cycle is estimated to be ∼30h [17], and a growth curve of DENV infection in Aag2 cells showed that DENV titers were increasing steadily at 48hpi, peaking only around 5 days pi (data not shown).
In our simple model context the time consumption of a replication event is affected by two factors in a linear manner: the first one b1 is invariant (representing the length-independent initiation/termination steps of the replication process); the other one, b2, is proportional to the length L of the sequence (this is the replication process itself).
In fact, analysis of the ARS305 region indicated that a major S-G1 differential signal extended for 2 2.5 kilobases on either side of the origin (Additional file 6: Figure S5B), coinciding with the previously described length of replication intermediates accumulated at HU-blocked replication forks [ 33, 35].
Length of fill, replication to working length, number of artificial depressions replicated, quality of replications, number of voids, and general appearance of obturation were all evaluated using standardized criteria.
The length of the sequence also affects replicability through the time required for completing replication.
Following the first round of amplification, a second round of amplification is performed using random primers that hybridize anywhere along the length of the transcript, and initiate 5' to 3' sequence replication.
We then measured the length of the two pulse-labels to obtain replication fork velocities, which were further classified into categories based on the distance travelled by the fork.
The length of the red stretches and the time of incubation allow the measurement of the replication rate.
Denoting the sequence length of replicators by N, the effective replication accuracy (Q e ) is obtained as by assuming that q and λ are uniform among the genotypes in x, that q is invariable over sequence positions, and that N is the same among the populations.
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