The total length of the fused silica capillary (i.e. 75 μm, PolyMicro, Phoenix, AZ, USA) was 67 cm; effective length to the detector was 60 cm.
Reactions were gel electrophorized; and single bands consistent with the expected length of the fused PCR product were obtained, purified, and diluted 100-fold before an additional round of PCR, isolation, purification, and dilution.
We have considered these fused filopodia as one object and have calculated the length of the fused filopodia using the method detailed above.
Therefore, the SHBG protein is only detected in LNCaP transfected cells, and the differing molecular weight of the immunoreactive bands was caused by the presence and length of the tags fused to the SHBG protein, which is an additional indicator of the specificity of the antibody.
This means that the length of the protein fused to pIII will vary, which should increase the likelihood of each signal peptide protein being efficiently displayed on the phage surface.
To be able to use emulsion haplotype fusion PCR to establish structural haplotypes, it was necessary both to increase the length of the amplicons fused in the emulsion (to allow the phasing of more SNPs simultaneously) and to simplify post-fusion detection of the phase.
The gun crew still had to gauge the best length of fuse for the range they were firing – ideally the shell should explode just before hitting its target.
Place an appropriate length of cannon fuse in the hole.
The length of the hairpin created by fusing the bacterial tracrRNA and crRNA can be varied by more than a dozen bases with equivalent activity (Esvelt et al., 2013 ), while tracrRNA equivalents from closely related bacteria can be substituted (Fonfara et al., 2013 ).
Finally, the outermost primers will exert their function in the next 30 cycles cycling program with lower annealing temperature and full length of multiple fuse DNA can be amplified (stage 5 in Figure 2).
